Moreover, a recent study revealed that recent PRN-deficient B. pertussis clinical isolates harboring ptxP3 variant and prn2 allele remain at higher CFUs/lung and are capable of sustaining infection longer than isolates still producing this adhesin, in mice immunized with a 3-component aP vaccine mice (Hegerle et al., 2014). Here, DHCR7-DT is linked to infection.