NPM1 and acute myeloid leukemia: In particular, Hussaini et al. (92), examining 187 subjects with a diagnosis of AML, highlighted the frequency of related-gene mutations, where ASXL1 was the highest mutated gene, followed by TET2, RUNX1, DNMT3A, TP53, IDH2, NRAS, FLT3, and NPM1. Moreover, NGS analysis identified co-mutated genes (ASXL1 with RUNX1 or TET2 or NRAS) and was able to discriminate somatic mutations associated with MDS/AML.