In an effort to develop an animal model that will facilitate the identification of immune markers and correlates of protection, and, in the long term, new immunotherapy strategies for ChD, in the present study, we analyzed whether experimental acute (10 and 30 days) and chronic (100 and 260 days) ChD alters the CD4+ Th1 and CD8+ Tc1 cell multifunctional capacities and inhibitory receptor co-expression on T cells in a murine model with a reticulotropic Y strain of T. cruzi. This evidence concerns the gene CD4 and coronary artery disorder.