To confirm the contribution of microglia to the clearance of synaptic material during retina degeneration in RCS rats, we eliminated microglia with the CSF1R inhibitor PLX3397 (600 ppm) (Supplementary Figures 3A–D), which was fed to 15-day-old RCS rats for 25 days (Supplementary Figure 3A). This evidence concerns the gene CSF1R and retinal degeneration.