We performed multiplex immuno-assay (Luminex) to assess the presence of inflammatory cytokines involved in the regulation NK cells function, such as TNF-α, IFN-γ, IL-6, IL-15, and IL-1β, in the tumor microenvironment (black bars, Figure 4A) and in the healthy adjacent tissue microenvironment (white bars, Figure 4A). This evidence concerns the gene TNF and neoplasm.