TNF and breast cancer: In parallel, in TNFα-stimulated spheroids of co-cultured MDA-MB-231 cells with breast cancer patient-derived CAFs, reduced ability to invade was revealed upon DAPT treatment (Figure 1C2); in addition, a marked change in the invasion pattern was noted after inhibition of the Notch pathway: The organized and directional motility of control cells (untreated by DAPT) has diverted into a dis-ordered and non-orchestrated phenotype in the presence of DAPT (Figure 1C1).