Of note, both LOB12.3-mIgG2a and LOB12.3-mIgG2a-DANA had the same weak anti-tumor effect: for LOB12.3-mIgG2a, the low efficacy is due to strong ADCC-mediated depletion by mIgG2a isotype; while for LOB12.3-mIgG2a-DANA, the lower efficacy is due to lack of FcγR-mediated crosslinking. Here, FCGR2A is linked to neoplasm.