On the contrary, it was observed that, after stimulation with anti-CD3 and anti-CD28, there was a significant increase in CD4+ T cells of a regulatory phenotype (CD25hiFoxP3+LAP+) in untreated psoriasis patients compared with the group treated with methotrexate (p = 0.03) (Fig. 4F). Here, TGFB1 is linked to psoriasis.