PKM and hepatocellular carcinoma: IF staining results were also similar to Fig. 2d, which showed that there was higher PKM2 expression located in both the cytoplasm and nucleus of NC HCC cells (Fig. 2e); However, PB2 treatment could not only reduce the fluorescence intensity of PKM2, but it could also inhibit the expression of PKM2 in the nucleus, which might be important for the inhibitory effects of PB2.