TNF and metabolic dysfunction-associated steatohepatitis: As TNFα was strongly upregulated in NASH liver, strongly correlated with ALT activity (HFD mice: rs = 0.666, p = 0.007, n = 15; 7 weeks MCDD mice: rs = 0.879, p = 0.002, n = 9) and well reported to mediate hepatocyte death27–30, we first evaluated the effect of the FSP27β overexpression on the sensitivity to cell death under basal conditions and in response to TNFα and TNFα with actinomycin D (Acti D).