The present study aimed to achieve the following: (1) to identify the genome-wide miRNA- and mastitis-related SNPs; (2) to confirm whether the candidate SNP in the seed region of bta-miR-2899 will affect the binding of bta-miR-2899 and the bovine SPI1 gene and its relationship with mastitis susceptibility. The gene discussed is SPI1; the disease is mastitis.