To study the expression of both macroH2A1 isoforms and their involvement in NAFLD, we considered two different mouse models of NAFLD/NASH/HCC [40]: a high fat diet (HFD)/diethylnistrosamine (DEN) mouse, and a phosphatase and tensin homolog (PTEN) liver-specific knock-out (KO) mouse [40]. Here, PTEN is linked to metabolic dysfunction-associated steatohepatitis.