Further demonstrating that LISP2- stained parasites are not artifact staining but bona fide constructive infections, parasites were stained positively with PV1 and similarly to the staining pattern observed in blood stages PV1 antibodies specifically highlight the circumference of the parasitophorous vacuole (Nyalwidhe and Lingelbach, 2006) (Figure 3c). This evidence concerns the gene PLVAP and infection.