Similar multi-tumor phenotype was also observed in a diethylnitrosamine (DEN)-induced carcinogenic liver injury model, in which significantly more, larger and multiple tumors with invasive or metastatic potential (displaying trabecular sinusoidal structures related to initial stage of invasion and metastasis in human hepatocellular carcinoma) were observed in the livers of the CYLD−/− mice as compared to that of the CYLD+/+ mice [68]. This evidence concerns the gene CYLD and hepatocellular carcinoma.