While in our case we considered the intra-tumoral infiltration region, and in Berthel et al. 32 the TME in <10 μm distance to the tumor was found to be relevant, both observations might capture the same effect as in our case the tumor border annotation was not highly precise (it was performed on the CD3-stained section and propagated to the CD8-stained section by co-registration) and mostly included the 10 μm distance. This evidence concerns the gene CD8A and neoplasm.