The results were further validated through study of OS index (LPx, lipid peroxidation) and antioxidant response, NFkB-mediated redox signaling and Ca2+ signaling as an index of cardiac contractility to have an in depth understanding of the efficacy of extraneous antioxidant supplementation (VIT-E and CRM) in altered thyroid status induced cardiac dysfunction. This evidence concerns the gene NFKB1 and thyroid gland disorder.