In this cross-sectional study, both NSE (threshold value of 40.9 ng/mL) and adjusted NSE (threshold value of 13.9 ng/mL) were significant in distinguishing PNET from the healthy group, whereas only adjusted NSE (threshold value of 25.4 ng/mL) was significant in a comparison of the PNET and benign groups. Here, ENO2 is linked to primitive neuroectodermal tumor.