Taken together, these data suggest that ODN2006 and M362 specifically induced a more phagocytic dendritic-cell-like maturation of THP-1 cells through the synergistic upregulation of TLR-9/-6 signaling pathways, leading to enhanced HLA class-I mediated activation of CD8+T cells and resulting in vaccine-mediated cytotoxicity on breast cancer cells. This evidence concerns the gene TLR9 and breast cancer.