As shown in Figure 1A, PARK2-PD cells displayed a significant increase of acidic compartments stained with LysoSensor DND-160 or with LysoTracker DND-99 and intensity quantification revealed about 3 and 1.5-fold change, respectively, for dyes staining in PARK2-PD cells compared to control cell lines (Figure 1B). This evidence concerns the gene PRKN and Parkinson disease.