In the early stages, more tumor metastasis‐associated proteins, such as beta‐2‐microglobulin (B2MG), alpha‐1‐acid glycoprotein (A1AG), galectin‐3‐binding protein (LG3BP), and macrophage colony‐stimulating factor 1 (CSF1), were identified in both the intracerebral and subcutaneous W256 models but showed no significant changes in the intracerebral C6 model. Here, CSF1 is linked to neoplasm.