AIF1 and ischemia: For analyses on microglia/macrophages, the allograft inflammatory factor 1 (Aif1), also known as ionized calcium-binding adapter molecule 1 (Iba1), was applied in combination with an endothelial marker, the platelet endothelial cell adhesion molecule (Pecam1 as cluster of differentiation 31, CD31), in the ischemia-affected neocortex.