Apart from these conclusive findings, an inconsistent pattern became further evident for neurofilaments as the present study—in terms of a completion of previous investigations related to the time point of 24 h after ischemia onset [27]—also failed to demonstrate an increased mRNA level for any of the neurofilaments investigated after 4 h of ischemia, which might be expected from the consistently detectable increased immunosignal of Nefl 24 h of ischemia. The gene discussed is NEFL; the disease is ischemia.