AGAP2 and hepatocellular carcinoma: To further investigate the functional roles of AGAP2-AS1 in HCC, we transfected Hep3B who had lowest expression of AGAP2-AS1 with functional pcDNA/AGAP2-AS1 and transfected HCCLM3 who had highest AGAP2-AS1 with specific shRNA (P < 0.01, respectively, Fig. 2a).