In addition, western blot demonstrated that ANXA11 was significantly up-regulated in HCC tissues compared with matched non-tumor tissues (P < 0.05, Fig.7j) Thus, we concluded that ANXA11 was a direct target of miR-16-5p and positively modulated by AGAP2-AS1 in HCC cells. The gene discussed is AGAP2; the disease is hepatocellular carcinoma.