In order to determine the expression pattern of RASSF1A in human healthy donor and PH patient (Idiopathic pulmonary arterial hypertension/IPAH, COPD-PH) lungs, we carried out laser-assisted micro-dissection of pulmonary vessels, followed by RNA isolation and real time PCRs with gene specific primers. This evidence concerns the gene RASSF1 and chronic obstructive pulmonary disease.