Figure 3B shows the results of Western blotting analysis for cellular proteins SERTAD1, CCND2, and HDAC3 expression in SFN-treated breast cancer cells. Each protein was quantified by measuring relative band intensities (Fig. 3C). Western blot analysis showed a noticeable decrease in the SERTAD1and CCDN2 in a dose-dependent pattern after incubation with SFN in ZR-75-1 cells; furthermore, HDAC3 expression was decreased in 25 μM SFN treatment (Fig. 3B, C). The gene discussed is CCND2; the disease is breast cancer.