PIK3CA and colorectal cancer: Colorectal cancers cells used in the study have a different mutation status in several critical genes involved in colorectal cancer in addition to BRAF (HCT-116 wt; HT-29 V600E) including TP53 (HCT- 116 wt; HT-29 mutation R273H), KRAS (HCT-116 mutation G13D; HT-29 wt), PIK3CA (HCT-116 H1047R; HT-29 wt) [53].