Figure 4A,B show that significant fluorescence signals were detected in cells exposed to SC F2. Furthermore, treatment of the ROS scavenger NAC inhibited the SC F2-induced ROS accumulation. We also observed that the level of catalase, an enzyme responsible for hydrogen peroxide degradation, decreased in SC F2-treated cells (Figure 4C). As shown in Figure 4D, the SC F2-induced PARP level in CRC cells was effectively blocked on co-culturing with NAC. This evidence concerns the gene PARP1 and colorectal carcinoma.