Figure 1E shows that SC F2 and TRAIL co-treated cells displayed more apoptotic morphology than cells treated with either substance alone (Figure 1E). We have confirmed similar results in HCT116, another CRC cell line (data not shown). Next, we examined the long-term effect of the SC F2 and TRAIL combination on clonogenic survival and observed that this combination proved to be effective in preventing colony formation (Figure 1F). As shown in Figure 1G, we found that SC F2 increased TRAIL-induced apoptosis in DLD-1 cells. The gene discussed is TNFSF10; the disease is colorectal carcinoma.