To further delineate the role of IL-17F on OTSCC cell invasion in a 3D spheroid model, HSC-3 or SCC-25 cells alone or in combination with cancer-associated fibroblasts (CAFs) were exposed to various concentrations of IL-17F in a Myogel/Collagen mixture for 5 subsequent days (Figure 6A). This evidence concerns the gene IL17F and cancer.