To identify new HIF-1α inhibitors, hepatocellular carcinoma cells (Hep3B) were stably transfected with p2.1, in which a hypoxia response element (HRE) from ENO1 gene was inserted upstream of firefly luciferase coding sequence and led to hypoxia-dependent firefly luciferase expression, and pSV-Renilla, in which renilla luciferase is consistently expressed. This evidence concerns the gene HIF1A and hepatocellular carcinoma.