Figure 7 shows the results obtained in PBMCs of PH1307 and PH1419, representative of an AC and a HAM/TSP patient, respectively. As control, PBMCs from non-infected individuals were also analyzed. As expected, in PBMCs of a healthy donor, THEMIS was expressed in the cytoplasm of a discrete percentage of cells (Figure 7, PBMC, Themis). The co-staining with DRAQ5 nuclear marker confirmed THEMIS as a cytoplasmic resident protein (Figure 7, PBMC, overlay). The gene discussed is THEMIS; the disease is tropical spastic paraparesis.