MCF-7 and MDA-MB-231 breast cancer cell lines were shown to display lower levels of mitochondrial membrane potential (MMP) and ATP production following NRF2 silencing [30], which are consistent phenomena to colon cancer cell lines, and therefore, these cell lines were chosen to ask whether NRF2 knockdown-mediated HIF-1α dysregulation is a general phenomenon throughout cell lines. This evidence concerns the gene NFE2L2 and malignant colon neoplasm.