In our research, we found retinoblastoma patients in high FEZF1-AS1 expression group had obviously shorter DFS compared with retinoblastoma patients in low FEZF1-AS1 expression group, and high FEZF1-AS1 expression was an independent unfavorable prognostic factor for DFS in retinoblastoma patients, which was similar to the prognostic value of FEZF1-AS1 expression in other types of human cancers. This evidence concerns the gene FEZF1 and retinoblastoma.