We found that the majority of embryos with morpholino injections exhibited abnormal heart or blood vessel phenotypes, not only in the CAD-associated atp2a2b group, but also in three of the four candidate gene groups, including the tram1, cypna1, and slc22a2 groups (Fig 4B and 4C), strongly implicating the association of these genes with CAD. This evidence concerns the gene TRAM1 and coronary artery disorder.