To determine if alterations in IL-6Rα expression and pSTAT3 activation were directly associated with hyperglycemia, primary neonatal mouse epidermal keratinocytes (MEKn) were cultured in normal glucose (5 mM glucose), hyperglycemic (25 mM glucose), or mannitol (5 mM glucose + 20 mM mannitol) media for 7 days. Here, IL6R is linked to Hyperglycemia.