TP53 and cancer: Thus, the most significantly downregulated canonical pathways observed in the double gene KO A2780s cell clones, when compared with the single gene KO cell clones, were related to the BRCA1 role in DNA damage response, the molecular mechanisms of cancer, p-53 signaling, and cell cycle regulation, in addition to major cancer-related signaling pathways such as AMPK signaling, and the mTOR signaling pathway (Figure 4(Aa)).