TSGA10, with the highest expression level in elongating spermatids and localized in the fibrous sheath of mature sperm, has previously been reported to be a biomarker in brain tumors28, 29 and regulated the development of esophageal squamous cell carcinoma.30AKAP4, as a kinase anchor protein, has been reported to contribute to the development of cervical cancer and ovarian serous carcinoma.31, 32 In this current study, we first provided evidence for the prognostic effect of TSGA10 and AKAP4 in the progression of TGCT, which may serve as potential treatment targets. Here, TSGA10 is linked to esophageal squamous cell carcinoma.