Building upon this, in this contribution, we aimed to mimic aspects of the tumor microenvironment by varying matrix density to achieve relevant “soft” and “stiff” moduli and incorporating different biomimetic integrin-binding peptides, specifically those derived from key ECM proteins collagen [(POG)3POGFOGER(POG)4], fibronectin/vitronectin (RGDS), and laminin (IKVAV). This evidence concerns the gene VTN and neoplasm.