Analysis of paracrine factors from different cell types revealed that VEGF secretion was more in supernatants from control hepatoma cells as compared to that observed in HBx-transfected cells while PDGF-BB and TGF-β levels were markedly enhanced in HBx-transfected hepatoma cells indicating that increased VEGF secretion may be inhibiting EMT while PDGF-BB and TGF-β may be a promoter of EMT phenotype. This evidence concerns the gene VEGFA and hepatocellular carcinoma.