PLP1 and Pelizeaus-Merzbacher spectrum disorder: To assess consequences of longer-term exposure to myelin debris, we incubated cultures for 1 or 7 days with myelin (0.1 mg myelin-protein ml-1) from wild type orPlp1 transgenic mice (Plp1 tg line #72;Readheadet al., 1994); the latter is a spontaneously demyelinating model of Pelizaeus Merzbacher disease (Andersonet al., 1999;Edgaret al., 2010) that like line #66 (Ipet al., 2006) is characterized by low-level T cell infiltration into the CNS (JE unpublished observations).