AkuNEC cells exhibited strong positivity for neuroendocrine tumor markers, such as neuron specific enolase (NSE), chromogranin A (CGA), cluster of differentiation 56 (neural cell adhesion molecule; CD56/NCAM), and synaptophysin (major synaptic vesicle protein p38; SYN/p38), but not somatostatin receptor (SSTR) and retinoblastoma (RB) protein by immunohistochemical (IHC) staining analysis, similar to the parental cell line (Table 1). This evidence concerns the gene ENO2 and neuroendocrine neoplasm.