As a crucial step for differential diagnosis of these autoimmune disorders, we determined specific subtypes of ANA in scurfy sera against the following targets: U1RNP, dsDNA (double stranded DNA), histone, Jo-1 (histidyl tRNA synthetase), ribosomal P protein, Sm (U2-U6 RNP), Scl-70 (topoisomerase I), PM-Scl (exosome complex), CENP-B (centromere protein B), PCNA (proliferating cell nuclear antigen), SSA/Ro60, Ro52/TRIM21 (tripartite motif proteins), SSB/La, centromere, RNA polymerase III, Rpp25, and Rpp38 (Th/To complex). This evidence concerns the gene TRIM21 and autoimmune disease.