To confirm the hepatoprotective effect of Baishouwu extract, the protein and mRNA expression levels of TLR4, MyD88, TRAF6, NF-κBp65, TGF-β1, and α-SMA in the livers were examined at inflammation, fibrosis and HCC stages in DEN-induced HCC model. This evidence concerns the gene TLR4 and hepatocellular carcinoma.