In the present work, the antinflammatory capability of Baishouwu extract mainly resulted in decreased levels of MyD88, TRAF6, NF-κB, IL-6 and TNF-α via the reduction of TLR4 expression in hepatitis, cirrhosis, and hepatocarcinoma stages of the HCC models induced by DEN. This evidence concerns the gene TLR4 and hepatitis A virus infection.