To confirm causality of the BCL11B p.R3S substitution for craniosynostosis and to generate a genetic model of the disorder, we introduced the c.7C>A mutation into the germ line of C57BL/6 mice using CRISPR-Cas9 (clustered, regularly interspaced, short palindromic repeat/CRISPR-associated protein 9) genome editing (40) as outlined in Figure 6 and described in the Reagents and Techniques section. The gene discussed is BCL11B; the disease is craniosynostosis.