Importantly, ROC curve analysis of our PAX7 target gene repression and the three DUX4 biomarkers on the single-cell RNA-Seq data from the van den Heuvel et al. (2019) (32) study demonstrated that PAX7 target gene repression was a powerful biomarker of FSHD myocytes, with an AUC of 0.651, compared to an AUC < 0.557 for the three DUX4 target gene biomarkers (Fig. 3E). This evidence concerns the gene DUX4 and facioscapulohumeral muscular dystrophy.