SPI‐1 plays an important role during the early phase of infection and enable salmonella to penetrate the intact intestinal epithelial barrier and reach the subepithelial tissue.36 In this study, syringaldehyde was identified as a novel inhibitor against SPI‐1 without impact on S. Typhimurium growth and cytotoxicity at the mmol/L range in vitro. Here, SPI1 is linked to infection.