Furthermore, RT‐PCR and western blotting assay revealed that CSC markers, including NANOG and OCT4, were decreased in the miR‐125b‐5p‐overexpressing hepatoma cells compared with the levels in the Negative and Blank Control cells (P < 0.01, Figure 2B and C; Figure S1A, Supporting Information). This evidence concerns the gene NANOG and hepatocellular carcinoma.