BRCA1 and ovarian carcinoma: Several studies demonstrated that NGS could be an efficient method for detecting somatic mutations in BRCA1 and BRCA2 genes using DNA from formalin-fixed and paraffin-embedded (FFPE) breast and ovarian cancer tissues (Ellison et al., 2015; Mafficini et al., 2016; Enyedi et al., 2016).