Colon-26 murine colorectal carcinoma cells, which express GSDMD but not ASC (Supplementary Fig. 1), were stably transfected with Fv3-mCasp1 (CL26-iCasp1 cells) or Fv3-mCasp8 (CL26-iCasp8 cells). This evidence concerns the gene PYCARD and colorectal carcinoma.