Further, qRT-PCR assay revealed that H19 as well as NAFLD-related genes (PPARγ, ACC1, SCD1, FASN and SREBP1) were significantly increased in the liver of HFD mice compared with control group (Figure 1D), while miR-130a was down-regulated in the liver of HFD mice. Here, FASN is linked to metabolic dysfunction-associated steatotic liver disease.