CD163 and hydrops fetalis: This differential expression was verified by real-time qPCR, which showed that the prenatal HF diet significantly altered hepatic mRNA expression of Cd163 and Hmgcr at 12 and 28 weeks of age, whereas expression of Aacs, Lpin1, Saa1, and Il1r1 was only significantly changed at week 12 (Fig. 4).