Hypomethylation of CASP8 was a risk factor for NTDs (aOR = 1.11; 95% CI, 1.05–1.17) according to the logistic regression model, which indicated that the risk for NTDs increased by 10.8% with every 1% reduction of the average methylation level of CASP8. Risk for two major subtypes of NTDs, namely, anencephaly and spina bifida, was also associated with hypomethylated CASP8 (Table 1). Here, CASP8 is linked to spina bifida.